Comparison of Chromogenic Culture Media, Rapid Immunochromatographic Test and Temocillin Resistance for The Detection of OXA-48 Carbapenemase-Positive Klebsiella Pneumonia Strains
Erkan Sanmak 1 * , Sebahat Aksaray 2
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1 Department of Medical Microbiology, Mardin State Hospital Medical Microbiology Laboratory, Mardin, Turkey
2 Department of Health Sciences, Haydarpasa Numune Training And Research Hospital, Istanbul, Turkey
* Corresponding Author

Abstract

Objectives: The study was aimed to compare the performance of three different approaches (temocillin disc diffusion test (TDD), immunochromatographic assay, OXA-48 K-SET, and chromogenic assay containing ChromID OXA-48 media) with PCR in a collection of K. pneumoniae producing OXA-48.
Materials and methods: A total of 45 carbapenem resistant K. pneumoniae isolates (27 were OXA-48 positive) were included in the study. Polymerase chain reaction (PCR), TDD, OXA-48 K-SET, ChromID OXA-48 media were applied to all isolates.
Results: The OXA-48 K-SET was found to be 100% compatible with gold standard PCR for the identification of OXA-48 positive Klebsiella pneumoniae strains. 100% sensitivity and 88.8% specificity were found with the ChromID OXA-48 medium method. Sensitivity and specificity of the TDD assay were found 100% and 77.8%, respectively.
Conclusion: The OXA-48 K-Set had excellent performance for detecting OXA-48 carbapenemase and can be used easily and reliably in the routine clinical microbiology laboratory for rapid detection of OXA-48 producing K. pneumoniae isolates, particularly in endemic regions. ChromID media and temocillin disc diffusion testing can be applied in laboratories as part of the identification and screening of carbapenemase-positive Klebsiella pneumoniae.

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This is an open access article distributed under the Creative Commons Attribution License (CC BY 4.0) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Article Type: Research Article

https://doi.org/10.29333/jcei/11267

J CLIN EXP INVEST, 2021 - Volume 12 Issue 4, Article No: em00778

Publication date: 14 Oct 2021

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Article Downloads: 98

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