The Importance of Serum Prolidase Activity in Endometriosis
Mukaddes Demir Dural, Fatma Devran Bıldırcın, Pervin Karlı, Ayse Zehra Özdemir
J Clin Exp Invest 2019;10(4):em00729.
https://doi.org/10.5799/jcei/5903
Research Article
[Abstract]
[PDF]
[References]
ABSTRACT
Objective: In this study, our objective was to evaluate the abnormal collagen destruction and turnover and the prolidase activity in the etiopathogenesis of endometriosis, which may deteriorate the collagenous structure of exracellular matrix (ECM).
Materials and Methods: For the assessment of the prolidase activity, venous blood samples were obtained from 37 patients, who had applied to the outpatient department of the Medical Faculty at Ondokuz Mayıs University with complaints of pelvic pain, dysmenorrhea and infertility between October 1st, 2011 and February 15th, 2012, underwent clinical and ultrasonographic examination, prediagnosed with endometriosis, and scheduled for laparoscopy or laparotomy. A total of 22 patients, who were diagnosed with endometriosis via intraoperative exploration and/or pathological examination, constituted the study group and the remaining 15 patients, who did not have any pathological finding or were diagnosed with a benign disease except for endometriosis, constituted the control group.
Serum samples obtained from all patients were first centrifuged and then stored in a freezer at -70 C until the time of analysis. During the analysis, the prolidase activity was measured following the necessary biochemical enzymatic processing.
Results: We found a statistically significant difference between stage 4 and stage 1,2,3 patients in the study group regarding the CA 125 levels (p=0.018). On the other hand, there was no statistically significant difference between stage 1,2,3 patients in the study group and the control group for serum prolidase levels (p=0.778). There was a statistically significant difference between stage 4 endometriosis patients in the study group and the control group considering the serum prolidase levels (p=0.026).
Conclusion: We conclude that serum prolidase activity has a critical function in the development of the endometriotic lesions. In endometriosis patients, the increase in the serum prolidase activity may play an important role in the progress to more advanced stages and in the development of infertility.
Keywords: serum prolidase activity, endometriosis, CA 125
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Do we really Need Lugol Solution in the Era of Energy Devices for the Preoperative Management of Patients with Graves’ Disease?
Kalin Nicolaev Vidinov, Yulian Stoinov
J Clin Exp Invest 2019;10(4):em00730.
https://doi.org/10.5799/jcei/5904
Research Article
[Abstract]
[PDF]
[References]
ABSTRACT
Introduction: Inorganic iodide (Lugol solution) has been used for the preoperative management of patients with Graves’ disease for many years. However, some new instruments have come into the surgeons reach that can accomplish bloodless operative field without the need of preoperative treatment. The purpose of this prospective study was to evaluate the efficacy and safety in the application of two types of energy devices – SonoSurg® and Thunderbeat® in patients with and without preoperative preparation with Lugol solution.
Material and Methods: The study included 100 patients with Greaves’ disease operated between January 2017 and December 2017. Patients were divided into four groups - 50 patients operated using SonoSurg® - 25 patients with preoperative preparation with Lugol solution and 25 without preoperative PI preparation, and 50 patients operated using Thunderbeat® - 25 patients with and 25 without preoperative Lugol preparation. We evaluated and compared the outcome between the four groups.
Results: The four groups had a similar distribution in the demographic features such as gender, age, and functional activity. When comparing the results, we found very similar results in the groups with preoperative preparation with PI solution. In contrasts we found significant differences in the duration of surgery, intraoperative and postoperative complications between the groups without preoperative PI preparation. Hospital stay of patients in four groups was similar - an average of 2 days stay into the clinic.
Conclusion: Our results showed that Lugol solution preparation has its place in the era of energy devices. Thunderbeat® can accomplish reliable hemostasis without preoperative Lugol preparation however we can achieve the same result with SonoSurg® and preoperative preparation at a much lower cost.
Keywords: Graves’ disease, Thunderbeat, Sonosurg, Lugol solution
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