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Abstract
Background: The benefits of vitamin C treatment for erectile functions during prepubertal crude oil-contaminated water (CCW) ingestion are unknown. We currently considered the import of vitamin C supplement during prepubertal crude oil-contaminated water ingestion on adult erectile mechanisms in Wistar rats.
Materials and methods: 18 prepubertal male rats were divided into three groups and given saline, 2.5 ml CCW, and 2.5 ml CCW + vitamin C (10 mg/kg), separately, until adulthood. Cavernosa tissue responses to phenylephrine, acetylcholine, potassium chloride, and responses after incubation with glibenclamide, nifedipine, methyl blue, and indomethacin were investigated. Serum testosterone and the cavernosa oxidative biomarkers were determined.
Results: Testosterone and catalase activity were significantly reduced in CCW group, while malondialdehyde activity was significantly increased. Potassium significantly increased cavernosa contraction in CCW-treated group. Incubation of the cavernosa with nifedipine and indomethacin reduced the percentage of relaxation in CCW group. When compared to the vitamin C-supplemented groups, incubating the cavernosa with methyl blue and glibenclamide markedly reduced relaxation in CCW.
Conclusions: Prepubertal CCW ingestion impaired adult cavernosa ATP-sensitive K+ channels, receptor and voltage-operated calcium channels, guanylate cyclase, and prostaglandin activity. Vitamin C administration reduced contractile impairments by increasing antioxidant activity in the cavernosa.
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Article Type: Research Article
J CLIN EXP INVEST, 2023, Volume 14, Issue 4, Article No: em00829
https://doi.org/10.29333/jcei/13751
Publication date: 02 Oct 2023
Article Views: 402
Article Downloads: 145
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