Objectives: Platelets are known blood cells differentiated from megakaryocytes in bone marrow and they play a key role in coagulation mechanism. They live several days in blood circulation and they aggregate in respond to stimulants such as thrombin, collagen, ADP (adenosine diphosphate) and epinephrine. Although platelets are known anucleated cells, it has been reported in vivo and in vitro that they undergo to apoptosis. In this study apoptotic effects of ADP on the platelets was investigated.
Materials and methods: P-selectin binding with flowcytometry, calcium levels with fluorometer, cytochrome-c levels with ELISA, caspase-3 gene expression with qRT-PCR, caspase-3 releasing with western blotting were determined in platelets.
Results: Platelets were incubated for 1 hour at 37°C with 10µM ADP. P-selectin binding, calcium and cytochrome-c levels, caspase-3 releasing and gene expression are significantly increased compared to control in ADP stimulated platelets.
Conclusions: Platelets can induce caspase-3 gene expression when stimulated with ADP which is a key enzyme plays an important role in apoptotic pathway.