A different insight into blood coagulation in vitro
Osman Evliyaoğlu 1 * , Selvi Kelekçi, M. Kemal Başaralı, Birgül Işık, Beri Hocaoğlu Bozarslan, Hanım Karahan
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1 Dicle Üniversitesi Tıp Fakültesi, Biyokimya Anabilim Dalı,Diyarbakır, Turkey
* Corresponding Author


Objectives: The known model of blood coagulation involves a series of zymogen activation reaction sequences. At each stage, a zymogen is converted to an active protease by cleavage of one or more peptide bonds in the precursor molecule. The aim of this study was to investigate amino acid profiles during coagulation process in different conditions in vitro.
Methods: Samples of serum and plasma (treated by EDTA or citrate) were obtained from healthy donors and from patients with Phenylketonuria (PKU). Amino acid profiles analyzed with reverse phase HPLC column.
Results: There were no differences between two plasma amino acid levels which were obtained by EDTA and acid citrate (p>0.05). Serum aspartate (asp), glutamate (glu), serine (ser), histidine (his) and phenylalanine (phe) levels were significantly higher in serum than plasma (p<0.05). This significant difference was not observed in patients with PKU.
Conclusion: As a result the enzymatic reactions of coagulation process generate some aminoacids and these reactions take place in an appropriate chemical microenvironment. This microenvironment can be used to clarify the stages of coagulation cascades with further studies.


This is an open access article distributed under the Creative Commons Attribution License (CC BY 4.0) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Article Type: Research Article


J Clin Exp Invest, 2010 - Volume 1 Issue 3, pp. 173-176

Publication date: 17 Dec 2010

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