The Investigation of Ameliorating Effect of Methylene Blue on Cisplatin-Induced Neurotoxicity in Female Rats
Ozlem Ozkul 1 , Bahattin Ozkul 2 * , Oytun Erbas 3
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1 Department of Medical Oncology, Bagcilar Research and Training Hospital, Istanbul, Turkey2 Department of Radiology, Istanbul Atlas University, Istanbul, Turkey3 Department of Physiology, Demiroglu Bilim University, Istanbul, Turkey * Corresponding Author


Objective: Cisplatin is indispensable in many solid tumors, and peripheral neurotoxicity is an essential dose-limiting side effect. The study aims to investigate the ameliorating effect of methylene blue, which has anti-oxidant properties, in rats with neurotoxicity due to cisplatin treatment.
Materials and methods: Twenty-four adult female rats were included in the study and divided into 3 groups. The first group (n=8), the control group, did not receive any treatment. The second (n=8) and the third (n=8) group received 2.5 mg/kg/day of cisplatin and 1 ml/kg/day of 0.9% NaCl (saline) twice a week for 4 weeks. Also, the third group received 20 mg/kg/day of methylene blue every day for 4 weeks. Blood samples were collected from rats for malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor- α (TNF-α), and interleukin-6 (IL-6) levels, and electromyography (EMG) and motor function were evaluated.
Results: In non-treated cisplatin injected rats, a significant increase in the lipid peroxidation product MDA and pro-inflammatory cytokines compared to the control group (p<0.001). Electromyography measurements also resulted in a significant decrease in cisplatin injected rats’ compound muscle action potential (CMAP) amplitude and prolongation in CMAP latency compared to the control group (p<0.05). A statistically significant decrease in MDA, TNF-α, and IL-6 levels and a statistically significant increase in GSH levels and CMAP amplitude was observed in the methylene blue-treated group compared to the group injected with cisplatin alone.
Conclusions: We concluded that methylene blue has ameliorating effects against cisplatin-induced neurotoxicity (CIN) by enhancing anti-oxidative capacity, energy metabolism and suppressing inflammatory parameters and oxidative stress.


This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Article Type: Research Article

J CLIN EXP INVEST, 2022, Volume 13, Issue 1, Article No: em00789

Publication date: 14 Jan 2022

Article Views: 984

Article Downloads: 375

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