The Importance of Serum Prolidase Activity in Endometriosis

Mukaddes Demir Dural, Fatma Devran Bıldırcın, Pervin Karlı, Ayse Zehra Özdemir

J Clin Exp Invest 2019;10(4):em00729.

https://doi.org/10.5799/jcei/5903

OPEN ACCESS

Download Full Text (PDF)

Abstract

Objective: In this study, our objective was to evaluate the abnormal collagen destruction and turnover and the prolidase activity in the etiopathogenesis of endometriosis, which may deteriorate the collagenous structure of exracellular matrix (ECM).
Materials and Methods: For the assessment of the prolidase activity, venous blood samples were obtained from 37 patients, who had applied to the outpatient department of the Medical Faculty at Ondokuz Mayıs University with complaints of pelvic pain, dysmenorrhea and infertility between October 1st, 2011 and February 15th, 2012, underwent clinical and ultrasonographic examination, prediagnosed with endometriosis, and scheduled for laparoscopy or laparotomy. A total of 22 patients, who were diagnosed with endometriosis via intraoperative exploration and/or pathological examination, constituted the study group and the remaining 15 patients, who did not have any pathological finding or were diagnosed with a benign disease except for endometriosis, constituted the control group.
Serum samples obtained from all patients were first centrifuged and then stored in a freezer at -70 C until the time of analysis. During the analysis, the prolidase activity was measured following the necessary biochemical enzymatic processing.
Results: We found a statistically significant difference between stage 4 and stage 1,2,3 patients in the study group regarding the CA 125 levels (p=0.018). On the other hand, there was no statistically significant difference between stage 1,2,3 patients in the study group and the control group for serum prolidase levels (p=0.778). There was a statistically significant difference between stage 4 endometriosis patients in the study group and the control group considering the serum prolidase levels (p=0.026).
Conclusion: We conclude that serum prolidase activity has a critical function in the development of the endometriotic lesions. In endometriosis patients, the increase in the serum prolidase activity may play an important role in the progress to more advanced stages and in the development of infertility.

Keywords

serum prolidase activity, endometriosis, CA 125

References

  • Sangi-Haghpeykar H, Poindexter AN 3rd. Epidemiology of endometriosis among parous women. Obstet Gynecol 1995;85:983.
  • Chatman DL, Ward AB. Endometriosis in adolescents. J Reprod Med 1982;27:156.
  • Missmer SA, Hankinson SE, Spiegelman D, et al. Incidence of laparoscopically confirmed endometriosis by demographic, anthropometric, and lifestyle factors. Am J Epidemiol 2004;160:784.
  • Lebovic DI, Mueller MD, Taylor RN. Immunobiology of endometriosis. Fertil Steril 2001;75:1.
  • Van Langendonckt A, Casanas-Roux F, Donnez J. Oxidative stress and peritoneal endometriosis. Fertil Steril 2002;77:861.
  • Harada T, Iwabe T, Terakawa N. Role of cytokines in endometriosis. Fertil Steril 2001; 76:1.
  • Eyster KM, Klinkova O, Kennedy V, Hansen KA. Whole genome deoxyribonucleic acid microarray analysis of gene expression in ectopic versus eutopic endometrium. Fertil Steril 2007;88:1505.
  • Sinaii N, Plumb K, Cotton L, et al. Differences in characteristics among 1,000 women with endometriosis based on extent of disease. Fertil Steril 2008;89:538.
  • Kennedy S, Bergqvist A, Chapron C, et al. ESHRE guideline for the diagnosis and treatment of endometriosis. Hum Reprod 2005;20:2698.
  • Engemise S, Gordon C, Konje JC. Endometriosis. BMJ 2010;340:c2168.
  • Hulboy DL, Rudolph LA, Matrisian LM. Matrix metalloproteinases as mediators of reproductive function. Mol Hum Reprod 1997;3:27-45.
  • Sillem M, Prifti S, Neher M, Runnebaum B. Extra cellular matrix remodelling in the endometrium and its possible relevance to the pathogenesis of endometriosis. Hum Reprod Update 1998;4:730-5.
  • Nap AW, Dunselman GA, de Goeij AF, Evers JL, Groothuis PG. Inhibiting MMP activity prevents the development of endometriosis in the chicken chorioallantoic membranemodel. Hum Reprod 2004;19:2180-7.
  • Collette T, Maheux R, Mailloux J, Akoum A. Increased expression of matrix metalloproteinase-9 in the eutopicendometrial tissue of women with endometriosis. Hum Reprod 2006;21:3059-67.
  • Arumugam K, Yip YC. De novo formation of adhesions inendometriosis: the role of iron and free radical reactions. Fertil Steril 1995;64:62-4.
  • Alpay Z, Saed GM, Diamond MP. Female infertility and freeradicals: potential role in adhesions and endometriosis. J Soc Gynecol Investig 2006;13:390-8.
  • Woessner JF Jr. Matrix metalloproteinases and theirinhibitors in connective tissue remodeling. FASEB J 1991;5:2145-54.
  • Murphy G. Matrix metalloproteinases and their inhibitors. Acta Orthop Scand Suppl 1995;266:55-60.
  • Vadillo-Ortega F, Gonzalez-Avilla G, Furth EE, et al. 92-kd type IV collagenase (matrix metalloproteinase-9) activity in human amniochorion increases with labor Am J Pathol expressed within the placenta during multiple stages of human pregnancy. Plasenta 1996;17:557.
  • Tsatas D, Baker MS, Rice GE. Differantial expression of proteases in human gestational tissues before, during and after spontaneous-onset labour at term. J Reprod Fertil 1999;116:43.
  • Chapron C, Fauconnier A, Dubuisson JB, et al. Deep infiltrating endometriosis: relation between severity of dysmenorrhoea and extent of disease. Hum Reprod 2003;18:760.
  • D’Hooghe TM, Debrock S, Hill JA, Meuleman C. Endometriosis and subfertility: is the relationship resolved? Semin Reprod Med 2003;21:243.
  • Mackenzie AP, Schatz F, Krikun G, et al. Mechanisms of abruption-induced premature rupture of the fetal membranes: Thrombin enhanced decidual matrix metalloproteinase-3 (stromeylsin-1) expression. Am J Obstet Gynecol 2004;191:1996.
  • Krikun G, Huang ST, Schatz F, et al. Thrombin activation of endometrial endothelial cells: a possible role in intrauterine growth restriction. Thromb Haemost 2007;97:245.
  • Burton GJ, Jauniaux E. Oxidative stres. Best Pract Res Clin Obstet Gynaecol 2011;25:287.
  • Chinard P. Photometric determination of proline and ornithine. J Biol Chem. 1952;199:61-65.
  • Onat T, Emerk K, Sözmen EY. Human Biochemistry, Palme publishing, Ankara, 2002.
  • Toy H, Camuzcuoglu H, Camuzcuoglu A, Celik H, Aksoy N. Decreased serum prolidase activity and increased oxidative stress in early pregnancy loss. Gynecol Obstet Invest 2010;69(2):122–12.
  • Camuzcuoglu H, Arioz DT, Toy H, Kurt S, Celik H, Erel O. Serum paraoxonase and arylesterase activities in patients with epithelial ovarian cancer. Gynecol Oncol 2009;112:481–5.
  • Koninckx PR, Kennedy SH, et al. Endometriotic disease: the role of peritoneal fluid. Hum Reprod Update 1998;4(5):741-51.
  • Hilali N, Vural M, Camuzcuoglu H, Camuzcuoglu A, Aksoy N. Increased prolidase activity and oxidative stress in PCOS. Clinical endocrinology 2013;79(1):105-110.
  • Vural M, Camuzcuoglu H, Toy H, Camuzcuoglu A, Aksoy N. Oxidative stress and prolidase activity in women with uterine fibroids. Journal of Obstetrics and Gynaecology 2012;32(1): 68-72.

Citation

Demir Dural M, Bıldırcın FD, Karlı P, Özdemir AZ. The Importance of Serum Prolidase Activity in Endometriosis. J Clin Exp Invest. 2019;10(4):em00729. https://doi.org/10.5799/jcei/5903