Osteogenic potency of human bone marrow mesenchymal stem cells from femoral atrophic non-union fracture site
Ismail Hadisoebroto Dilogo, Phedy Phedy, Erica Kholinne, Yoshi Pratama Djaja 1 * , Yuyus Kusnadi, Lakshmi Sandhow
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1 Dept. Orthopaedic and Traumatology, Faculty of Medicine Universitas Indonesia* Corresponding Author

Abstract

Objective: Mesenchymal stem cells (MSCs) exist in the site of atrophic non-union fracture. The aim of this study was to evaluate the osteogenic potency of MSCs in order to have a better understanding of the unclear pathophysiology of atrophic non-union fracture
Methods: This is an in vitro experimental study. Sample was obtained from the non-union site of a patient with a 6-years-history of atrophic non-union fracture of right femur. The MSCs was isolated from the fracture site and was cultured in the growth medium. Confirmation of the MSCs was performed and then osteogenic differentiation was performed in mono-layered MSC grown in both home-made and commercial osteogenic media. To evaluate the osteogenic differentiation, we performed Alizarin red staining and colorimetric assay for alkaline phosphatase (ALP).
Results: From Alizarin red staining, most cells in the osteoblast medium were stained red by the staining. The result of colorimetric assessment of ALP shows that peak concentration was reached after 4 minutes in osteogenic group and control group.
Conclusion: The presence of ALP activity and positive Alizarin red staining in our study showed that MSCs stem cells obtained from site of atrophic non-union is capable to be differentiated into osteogenic cells.

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Article Type: Research Article

J Clin Exp Invest, 2014, Volume 5, Issue 2, 159-163

https://doi.org/10.5799/ahinjs.01.2014.02.0382

Publication date: 11 Jun 2014

Article Views: 2122

Article Downloads: 951

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