Objective: As the external auditory canal is a moisture area, it facilitates the growth of bacteria and fungi. Infections and inflammation due to Staphylococcus aureus, Pseudomonas aeruginosa, Aspergillus spp. and Candida albicans can develop in this area. Classical Castellani solution including boric acid, fenol, fucsin, resorcinol, acetone, and alcohol is used for external ear tract infections due to fungi and bacteria, and also for the superficial dermatophytoses, and eczematous dermatitis of the external ear tract infections.
The purpose of this study is to investigate of the in vitro effectiveness of classical Castellani solution and its different formulations with different dilutions against the standard yeast and bacteria strains.
Methods: C. albicans ATCC 10231, C. krusei ATCC 6258, C. dubliniensis CD 36, C. guilliermondii ATCC 6260, C. parapsilosis ATCC22019, E. coli ATCC 25922, P. aeruginosa ATCC 27853, MRSA ATCC 43300, Staphylococcus aureus ATCC 25923, and S. epidermidis ATCC 12228 strains were included in the study. Broth microdilution method was used for each microorganism and Castellani formulation. The tests are repeated at least twice.
Results: The inhibitory concentration of classical Castellani solution against bacteria and fungi is 1/64-1/256, 1/32-1/64 for fuchsin free solution, 1/32-1/128 for boric acid-free solution and, 1/64-1/128 for resorcinol-free solution.
Conclusions: As a conclusion we think that the classical Castellani solution and its different formulations at various dilutions may be effective antimicrobial agents for different patient populations.