Detection of MYC gene rearrangements by conventional cytogenetics and fluorescent in situ hybridization in patients with acute lymphoblastic leukemia cases
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Seda Eren, Ayşe Çırakoğlu 1 * , Dilhan Kuru, Zafer Başlar, Ömer Devecioğlu, Meliha Nalçacı, Seniha Hacıhanefioğlu
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1 İstanbul Üniversitesi, Cerrahpaşa Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, Turkey
* Corresponding Author

Abstract

Objective: The aim of this study is to investigate rearrangements at the region of MYC gene by conventional cytogenetics and interphase FISH methods in patients with acute lymphoblastic leukemia (ALL).
Methods: The study was carried out on bone marrow specimens of 25 ALL patients who were referred to our laboratory. Fourteen children and 11 adult ALL cases were examined. Conventional cytogenetic analysis was performed using G banding technique and fluorescence in situ hybridization technique was applied using MYC breakapart probe (Cytocell).
Results: Totally, in 2 of 25 cases available metaphases were not obtained. While in 9 of 23 cases were found to have normal karyotype (39.1%), numerical chromosomal abnormalities were detected in 6 cases, structural abnormalities in 4 and both numerical and structural abnormalities were found in 4 cases.
In one case (4%) t(8;14)(q24;q32) was found as a cytogenetic aberration in which MYC gene locus involved. FISH analysis was performed successfully in all cases and MYC rearrangements were found in 3 cases (12%) by FISH method.
Conclusion: By comparing two techniques, it was observed that FISH method showed more sensitivity, however conventional cytogenetic techniques were also effective to reveal all changes of the chromosomes. Therefore, we concluded that it would be more efficient to use of these two techniques together.

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Article Type: Research Article

https://doi.org/10.5799/ahinjs.01.2015.01.0480

J Clin Exp Invest, 2015 - Volume 6 Issue 1, pp. 21-26

Publication date: 10 May 2015

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Article Downloads: 494

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