Comparison of two different methods for the determination of erythrocyte sedimentation rate
Gülfer Öztürk, Şeyda Özdemir 1 * , Aynur Altuntaş, Sümeyya Akyol, Erdem Bulut, Gönül Erden, İsmail Temel
More Detail
1 Dışkapı Yıldırım Beyazıt Education /Research Hospital, Dept. Biochemistry, Ankara, Turkey* Corresponding Author

Abstract

Objective: Erythrocyte Sedimentation Rate (ESR) can be used for the follow-up of number of diseases. In recent years, closed automated systems that measure ESR directly from a capped EDTA and citrate blood sample tube have been developed. In this study, we aimed to compare and evaluate the consistence of assay results of iSed Alcor Auto-instrument and Berkhun SDM60 Auto-instrument.
Methods: K2EDTA and citrated blood samples were taken from 149 randomly selected outpatients. The ESR of blood samples in tubes containing K2EDTA was determined by iSed Alcor Auto-instrument whereas Berkhun SDM60 Auto-instrument was used to determine the ESR of blood samples in tubes containing citrate.
Results: The mean ± SD ESR was 24.48 ± 23.10 mm/hr (95% CI for the mean was 20.54–28.02 mm/hr) for iSed Alcor Auto-instrument and 23.94 ± 17.24 mm/hr (95% CI for the mean was 21.15–26.73 mm/hr) for the Berkhun Auto-instrument. We found the mean difference between two methods as 0.336 mm/hr (95% CI for the mean was −2.06 to 1.39 mm/hr; P = 0.701). The obtained correlation coefficient was 0.90 (P = 0.0001). There was no evidence of systemic bias, equal to 0.3 mm/hr (limits of agreement, –20.6 to 21.2 mm/hr).
Conclusion: iSed Alcor Auto-instrument and Berkhun SDM60 Auto-instrument might be used as alternative systems each other. However, it should be monitored carefully in especially high ESR (>50 mm/hr) results and the results should be checked according to the Westergren method.

License

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Article Type: Research Article

J Clin Exp Invest, 2014, Volume 5, Issue 3, 371-375

https://doi.org/10.5799/ahinjs.01.2014.03.0422

Publication date: 09 Sep 2014

Article Views: 3182

Article Downloads: 1156

Open Access References How to cite this article