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Abstract - A Single Nucleotide Polymorphism of Follicle-Stimulating Hormone Receptor Gene in Turkish Women with PCOS: A Cross-Sectional Study
Ümit Naykı, Altuğ Koç, Cenk Naykı, Özge Özer Kaya, Mehmet Kulhan, Murat Günay

Objective: The aim of this study was to evaluate both the frequency of Asn680Ser polymorphism of follicle stimulating hormone receptor (FSHR) and whether this polymorphism is associated with PCOS in a Turkish population.

Patients and Methods: 100 patients with PCOS and 100 controls were recruited from Eastern Turkey. Venous blood samples were obtained for genetic study, hormone profile, glucose and insulin assays. Asn680Ser polymorphism of FSHR was analyzed by polymerase chain reaction (PCR) amplification and direct DNA sequencing. Two groups were compared for body mass index (BMI), modified Ferriman-Gallwey (mFG) score, fasting glucose, fasting insulin, insulin resistance, and hormone and lipid status. Then, all patients were divided into subgroups for genotypes of FSHR polymorphism. These subgroups were also compared for these mentioned parameters.

Results: The difference between women with PCOS and controls in allelic frequencies for Asn680Ser polymorphism of FSHR were not significant.  When this single nucleotide polymorphism (SNP) at codon 680 was separately analyzed, no significant difference was found in the genotype frequency among the Asn680Asn, Asn680Ser, andSer680Ser subgroups. There were no significant differences in BMI, mFG score, levels of fasting glucose and fasting insulin, HOMA-IR, and also levels of FSH, LH, E2, PRL, TSH and TT among the PCOS patients carrying different genotypes of Asn680Ser polymorphism

Conclusion: there is no association between Asn680Ser polymorphism of FSHR and PCOS in Turkish women from eastern provinces of Turkey. In addition, this SNP seems not to be associated with BMI, IR and PCOS-associated endocrine hormones.

Key words: Polycystic ovary syndrome, follicle stimulating hormone receptor, single nucleotide polymorphism


J Clin Exp Invest

DOI: 10.5799/ahinjs.01.2017.02.0620

Volume 8, Number 2 (2017)